Opinion for the court filed by Circuit Judge CLEVENGER. Dissenting opinion filed by Circuit Judge PAULINE NEWMAN.
Housey Pharmaceuticals, Inc. (“Hous-ey”) sued Astrazeneca UK Ltd., Astraz-eneca LP, Astrazeneca Pharmaceuticals LP, Aventis Pharmaceuticals, Inc., Bristol-Meyers Squibb Co., Merck & Co., Inc., Roche Holdings, Inc., Hoffman-La Roche Inc., Roche Laboratories, Inc., Syntex (U.S.A.) Inc., Wyeth, and Wyeth Pharmaceuticals, Inc. (collectively “defendants”) in the United States District Court for the District of Delaware, alleging infringement of four patents addressing methods of screening for protein inhibitors and activators. The district court construed several of the limitations of the patent claims, including “inhibitor or activator of a protein.” Bayer Ag v. Housey Pharm., Inc., No. 01-148-SLR, 2002 WL 31833205 (D.Del. Nov. 12, 2002)1. Housey subsequently stipulated that, if this construction were not reversed or modified on appeal, its patents would be invalid and not infringed. Housey Pharm., Inc. v. Abbott *1350Pharm., Inc., No. 01-401-SLR (D.Del. Nov.26, 2002). The district court ordered a final judgment of invalidity and nonin-fringement. Housey Pharm,., Inc. v. Abbott Pharm., Inc., No. 01-401 SLR (D.Del. Nov.27, 2002). We conclude that the district court’s construction of “inhibitor or activator of a protein” was not erroneous, and we therefore affirm the judgment.
I
Housey is the owner by assignment of U.S. Patents No. 4,980,281, No. 5,266,464, No. 5,688,655, and No. 5,877,007, all of which are titled “Method of Screening for Protein Inhibitors or Activators.” Concerning the limitation “inhibitor or activator of a protein,” the parties treat the use of the limitation in claim 1 of the '281 patent as representative, and we do likewise:
1. A method of determining whether a substance is an inhibitor or activator of a protein whose production by a cell evokes a responsive change in a pheno-typic characteristic other than the level of said protein in said cell per se, which comprises:
(a) providing a first cell line which produces said protein and exhibits said phenotypic response to the protein;
(b) providing a second cell line which produces the protein at a lower level than the first cell line, or does not produce[ ] the protein at all, and which exhibits said pheno-typic response to the protein to a lesser degree or not at all;
(c) incubating the substance with the first and second cell lines; and
(d) comparing the phenotypic response of the first cell line to the substance with the phenotypic response of the second cell line to the substance.
’281 patent, col. 24, ll. 46-63 (emphasis added).
The patented method is an assay, or test, to determine whether a substance, often a candidate for a pharmaceutical product or drug, is an “inhibitor or activator” of a particular protein (the “protein of interest” or “POI”) in a cell. Steps (a) and (b) of the method require the existence of two different cell lines. One cell line produces more of the POI, and the other produces less, or none, of the POI. (The cell line producing more of the POI is sometimes referred to as the “test” cell line, and the cell line producing less or none of the POI is sometimes referred to as the “control” cell line.) Additionally, the two cell lines are differentiated by the fact that the increase in the amount of the POI in one of the cell lines modifies some characteristic in that cell line. In the language of claim 1 of the '281 patent, the relevant characteristic of each of the cell lines is labeled a “phenotypic response” to a different concentration of the POI, and the difference between the relevant characteristic in the two cell lines (presumably caused by the increase in the POI) is a “responsive change in a phenotypic characteristic.” In step (c) of the method, the substance being screened is added to each of the cell lines. Step (d) requires comparing the effect that the substance has on the phenotypic response to the POI in each cell line.
In Housey’s words, the method requires treating the two cells “with a test substance to determine whether that substance inhibits the cellular functioning of the POI ... as measured by modulations of the responsive change in the phenotypic characteristic of [one] cell, compared with the modulations of the same responsive phenotypic characteristic, if any, in the [other] cell.” The method is allegedly valuable because if the substance being *1351screened impacts the phenotypic response of the cell with an increased concentration of the POI and does not affect the cell containing less or none of the POI in the same way, a logical guess is that the substance has this differential impact because the substance has an effect on the POI. This knowledge is valuable: If a POI is believed to be implicated in a certain disease, and a substance is known to affect that POI, then the substance is more likely to have some value in treating the disease than another substance which does not affect the biological activity of the POI.
II
The district court conducted a Mark-man hearing and construed five claim terms disputed by the parties, including “inhibitor or activator of a protein,”2 which was held to mean “a substance that has a greater effect on the phenotype of cells that express the protein of interest at a higher level than on the phenotype of cells that express the protein of interest at a lower level or not at all.”
The district court considered and expressly rejected House/s argument that an “activator or inhibitor of a protein” was limited to substances that “directly interact [with], ie., bind to, the target protein.” The interpretation adopted by the district court includes within an “inhibitor or activator of a protein” substances that affect the test cell’s “responsive change in a phe-notypic characteristic” through the direct-binding concept to which Housey argues the term is absolutely limited. In addition, however, it is broader: it includes substances that operate through other indirect concepts of molecular interaction. For example, under the district court’s interpretation, a substance being screened may still be an “inhibitor or activator of a protein” if it binds to and alters the metabolic functioning of a molecule other than the POI that is essential to either a biochemical pathway required to produce the POI in the first place or a biochemical pathway in which the fully formed POI participates in the cell in order to trigger the “pheno-typic response.” In other words, the district court’s definition includes within the scope of an “inhibitor or activator of a protein” substances that inhibit or activate the biological activity of the POI in the cell without binding to the POI itself.
Housey stipulated that, “based upon the Court’s construction of the claim limitation ‘inhibitor or activator of a protein,’ ... [and] subject to its right to appeal the Court’s claim construction and any final judgment based thereon, that the Court may enter an order stating that the patents in suit are invalid and not infringed.” Housey and the defendants, however, “reserve[d] all of their claims, counterclaims, arguments and defenses” if “the Court’s determinations on ... issues of claim construction [related to ‘inhibitor or activator of a protein’] are reversed or modified on appeal such that the matter is remanded for further consideration.” Based on the stipulation, the district court entered final judgment in favor of the defendants.
Housey appealed to us, and we have jurisdiction to hear the appeal pursuant to 28 U.S.C. § 1295(a)(1).
Ill
We review the district court’s claim construction de novo. Cybor Corp. v. FAS Techs., Inc., 138 F.3d 1448, 1456 (Fed.Cir.1998) (en banc). Claim construction begins with the language of the *1352claims. Johnson Worldwide Assocs., Inc. v. Zebco Corp., 175 F.3d 985, 989 (Fed.Cir.1999). When construing patent claims, there is a “heavy presumption” that the language in the claim “carries its ordinary and customary meaning,” CCS Fitness, Inc. v. Brunswick Corp., 288 F.3d 1359, 1366 (Fed.Cir.2002), “amongst artisans of ordinary skill in the relevant art at the time of the invention,” ResQNet.com, Inc. v. Lansa, Inc., 346 F.3d 1374, 1378 (Fed.Cir.2003). Dictionaries and treatises may provide insight into a term’s ordinary meaning thus defined. Tex. Digital Sys., Inc. v. Telegenix, Inc., 308 F.3d 1193, 1202-03 (Fed.Cir.2002). However, a claim must also be considered in the context of the intrinsic evidence, namely the claims, the specification, and the prosecution history. Rexnord Corp. v. Laitram Corp., 274 F.3d 1336, 1342-43 (Fed.Cir.2001). If there is a discernable plain and ordinary meaning of the claim language, then this meaning usually defines the scope of the claims unless the patentee has explicitly disclaimed or clearly disavowed this meaning in the specification or prosecution history. Liebel-Flarsheim Co. v. Medrad, Inc., 358 F.3d 898, 906 (Fed.Cir.2004) (requiring “words or expressions of manifest exclusion or restriction” before broad terms in a claim will be read narrowly in light of a narrow specification (quoting Teleflex, Inc. v. Ficosa N. Am. Corp., 299 F.3d 1313, 1327 (Fed.Cir.2002)); Middleton, Inc. v. Minn. Mining Mfg. Co., 311 F.3d 1384, 1388 (Fed.Cir.2002) (This court also considers the prosecution history ... to determine whether the applicant clearly and unambiguously ‘disclaimed or disavowed [any interpretation] during prosecution in order to obtain claim allowance.’) (quoting Standard Oil Co. v. Am. Cyanamid Co., 774 F.2d 448, 452 (Fed.Cir.1985) (alteration in original))). For example, the patentee may act as his own lexicographer and expressly define the term in the specification. See Tex. Digital Sys., 308 F.3d at 1204; Rexnord, 274 F.3d at 1342.
A
The language of claim 1 of the '281 patent clearly supports the district court’s construction and is inimical to any narrower construction. The claim recites “[a] method of determining whether a substance is an inhibitor or activator of a protein.” '281 patent, col. 24, ll. 46-47. The steps of the method in turn permit a determination of whether substances affect phenotypic response to the POI in the test and control cells differently. Thus, if “a substance ... has a greater effect on the phenotype of cells that express the protein of interest at a higher level than on the phenotype of cells that express the protein of interest at a lower level or not at all,” the substance is, as defined by the claimed method itself, an inhibitor or activator of the protein of interest.
Furthermore, the specification expressly defines an “inhibitor or activator of a protein” as a substance that yields a positive response to the claimed method, just as the district court did. See id. at col. 3, ll. 5-10 (“Substances which specifically inhibit or inactivate [sic] the POI may be distinguished from substances which affect cell morphology or growth by other mechanisms in that they will have a greater effect on the test lines than on the control line.”); id. at col. 4, ll. 48-50 (“Inhibitors or activators are identified by their greater effect on the phenotype of the higher producing cell line.”).
Housey argues that the district court’s construction “confuses the method of distinguishing activators or inhibitors with the definition of activators or inhibitors.” We disagree. The claim addresses a method of determining whether a compound is an inhibitor or activator that is conducted by observing whether a particu*1353lar event occurs when the method is performed. It is therefore logical to construe an “inhibitor or activator of a protein” as a substance that precipitates that event, especially given that the specification, too, defines an inhibitor or activator in the same way.
B
Housey’s principal argument on appeal with respect to the construction of “inhibitor or activator of a protein” is the same one that was rejected by the district court: Housey argues that this limitation is “properly construed to mean substances that interact with, ie., bind to, the POI in order to exert their inhibiting or activating effect on the cellular functioning of the POI.” We do not agree that, as used in the '281 patent, an “inhibitor or activator of a protein” is limited to substances that achieve their effect on the biological activity of the POI through a particular reaction mechanism, namely binding to the POI. A substance need only have “a greater effect on the phenotype of cells that express the protein of interest at a higher level than on the phenotype of cells that express the protein of interest at a lower level or not at all” to be an “inhibitor or activator of a protein,” and an inhibitor or activator of a POI may alter the biological activity of the POI through a direct POI-binding mechanism or an indirect pathway-binding mechanism. The word “bind” or “binding” appears nowhere in the '281 patent in conjunction with an “inhibitor or activator of a protein.”
At oral argument (and thus on the late side of things), Housey produced definitions of “inhibitor” and “activator” from technical dictionaries to argue for the first time on appeal that the plain meaning of an “inhibitor or activator of a protein” is limited to a substance that binds to the protein. With respect to an inhibitor or activator of an enzyme, these definitions do include a direct-binding limitation. See Chambers Dictionary of Science and Technology 13 (1999) (defining “activator” as “[o]f an enzyme, a small molecule which binds to it and increases its activity”); Henderson’s Dictionary of Biological Terms 284 (11th ed.1995) (defining “inhibitor” as “a substance which ... prevents the normal action of an enzyme ... by binding to the active site ... [or] by binding to other parts of the enzyme”). However, the claim language is an “inhibitor or activator of a protein,” not of an enzyme,3 and the dictionaries also include a more appropriate general definition of both “activator” and “inhibitor” that requires only an effect on the biological activity of the protein, regardless of the mechanism used to achieve that effect. See Chambers Dictionary of Science and Technology 13 (defining “activator” as “[a]ny agent ... whose presence stimulates biochemical or physiological changes”); Henderson’s Dictionary of Biological Terms 284 (defining “inhibitor” as “any agent which checks or prevents an action or process”). Thus, according to the evidence submitted by Housey, the plain meaning of “inhibitor or activator of a protein” does not support the inclusion of the direct-binding concept as a limitation on the claimed method.
The intrinsic evidence of the '281 patent does not clearly disavow this broad plain meaning. In fact, even if the plain meaning were considered to be ambiguous, supporting both a narrower definition limited to inhibitors and activators that bind to the POI and a broader definition of inhibitors and activators that need only affect the biological activity of the POI, the district *1354court was correct because the specification and prosecution history affirmatively demonstrate that Housey intended the broader meaning that is not limited to direct binding.
The specification foresaw the possibility of a substance affecting the “responsive change in a phenotypic characteristic” in the cell line with the greater amount of POI without the substance binding to the POI. See '281 patent, col. 6, ll. 29-33 (“[C]ell lines will be sensitive in their growth properties [i.e. in the their relevant phenotypic characteristic] to chemical agents which are capable of binding to, or modifying the biological effects of, the POI.” (emphasis added)).4 The specification also expressly defines the terms “inhibitors” and “activators” in terms of the biological activity of the POI 40-42 (“The term ‘inhibitors’ includes both substance[s] which reduce the activity of the POI and th[o]se which nullify it altogether.”); id. at ll. 37-40 (“The term ‘activators,’ as used herein, includes both substances necessary for the POI to become active in the first place, and substances which merely accentuate its activity.”).
Housey’s statements in the prosecution history of the '281 patent also support this conclusion. In distinguishing prior art during prosecution, Housey repeatedly characterized his invention as one that could determine whether a substance interacted with the biological pathway in which a particular protein functioned: The prior art did “not provide the target protein/biochemical pathway specificity of the present invention.” Because biological pathways usually involve more than one protein, a substance can clearly interact with the POI’s biological pathway without binding with the POI itself, and Housey stated as much in the process of distinguishing another prior art reference:
Substances ... identified or characterized using the teachings of the Housey patents will be found to interact directly with the target protein itself and/or with other proteins in which the target protein also interacts in the form of dimeric or multimeric complexes. In this fashion, the approach disclosed in the Hous-ey patents provides a specificity between a target protein and its attendant biochemical pathways within the cell that is impossible to achieve with other assay systems.
Housey argues that an inhibitor or activator is limited to substances that bind to the POI because the claim language specifies an inhibitor or activator “of a protein.” The fact that the biological activity “of a protein” is affected by a screened substance, however, lends no insight into whether the effect must be attributed to binding to the protein or some other type of influence on the biological pathways through which the protein is created and in which the protein functions.
Similarly, Housey argues that the recurring presence of the terms “specific” and “specifically” in the specification and the prosecution history conclusively demonstrates that the inhibitor or activator operates by binding with the POI. See, e.g., '281 patent, col. 1, ll. 10-12 (“In particular, [the invention] is concerned with a method of screening for substances which specifically inhibit or activate a particular protein.” (emphasis added)); id. at col. 2, ll. 27-28 (describing the invention as having a “specificity for detecting *1355an active agent exceeding that of’ the prior art assays); id. at ll. 28-32 (“[T]he method which we describe herein involves the generation of a cell line purposefully engineered to detect both stimulatory and inhibitory agents which are absolutely specific for any given protein which affects the cultural of morphological characteristics of a cell.” (emphasis added)); id. at col. 9, ll. 30-31 (stating that the method was screening for “substances which may contain biologically active agents specific to the POI” (emphasis added)). Leaving aside the absence of the word “specific” in the claim language (and our repeated warnings not to import limitations from the specification that are not present in the claims, Amgen Inc. v. Hoechst Marion Roussel, Inc., 314 F.3d 1313, 1325 (Fed.Cir.2003) (“The danger of improperly importing a limitation is even greater when the purported limitation is based upon a term not appearing in the claim.”)), this language from the specification does not lead to a strong inference of direct binding to the POI. As the above-quoted sections of the prosecution history demonstrate, the use of “specific” suggests equally as strongly that the biological activity of the POI is affected, through a direct or indirect mechanism, when the biological activity of other proteins in other biochemical pathways is not. Furthermore, the specification, too, equates specificity with the biological effect of the inhibitor or activator, not with any particular mechanism of action. See '281 patent, col. 3, ll. 5-10 (“Substances which specifically inhibit or inactivate the POI may be distinguished from substances which affect cell morphology or growth by other mechanisms in that they will have a greater effect on the test lines than on the control line.” (emphasis added)).5
Finally, Housey points to language at the end of one of the specific embodiments in the specification and argues that it demonstrates that the patented assay must be able to detect substances that bind to the POI: “[T]his work establishes, for the first time, the fact that stable overproduction of a protein in mammalian cells can result in a novel cellular phenotype(s) ... which can be directly modulated by chemical agents which interact with the protein.” Id. at col. 18, ll. 30-36 (emphasis added). First, this passage does not indicate that the interaction between the agent being screened and the POI must be in the form of direct binding; another protein or cellular component may provide the mechanism through which the agent interacts with the POI. Second, not only does this passage describe only a single embodiment (and limitations on specific embodiments are not to be imported into the claims, see Electro Med. Sys., S.A. v. Cooper Life Scis., Inc., 34 F.3d 1048, 1054 (Fed.Cir.1994)), but it describes an embodiment in which there is “a direct relationship between the molar amount of inhibitor [or activator] required to prevent [or stimulate] [the phenotypic response] and the molar amount of [the POI] present in each cell line.” '281 patent, col. 18, ll. 22-24, 28-30. This additional fact notes a direct relationship between the amount of POI overexpressed in the test cell and the amount of inhibitor or activator required to eliminate the “responsive change in a phenotypic characteristic.” If true, this fact may or may not permit one of skill in the art to use the claimed method to divine that the inhibitor or activator binds directly to the POI, but it unquestionably is not *1356a claimed limitation in the “method of determining whether a substance is an inhibitor or activator of a protein” of claim 1 of the '281 patent.
C
Housey invokes two canons of claim construction to support limiting an “inhibitor or activator of a protein” to a substance that binds to that protein, but neither is persuasive. First, Housey cites Athletic Alternatives, Inc. v. Prince Manfacturing, Inc., 73 F.3d 1573, 1581 (Fed.Cir.1996), for the proposition that in the event of a tie between a broader and narrower meaning, the notice function of patent law requires that the narrower meaning prevails. Housey misreads Athletic Alternatives. In that unusual case, one patent applicant made two contradictory and irreconcilable affirmative representations of the contested limitation. In those circumstances, we held that the narrower interpretation trumps the broader interpretation. In this case, there are no contradictory and irreconcilable choices for the meaning of “inhibitor or activator of a protein.” Second, Housey relies on the axiom that “[e]laims should be so construed, if possible, as to sustain their validity.” Rhine v. Casio, Inc., 183 F.3d 1342, 1345 (Fed.Cir.1999) (quoting Carman Indus., Inc. v. Wahl, 724 F.2d 932, 937 n. 5 (Fed.Cir.1983)). Here, we have not been asked to review validity, and the district court did not pass on validity. Housey’s stipulation of invalidity alone is insufficient to invoke this canon. In any event, the canon lacks dispositive effect in this case. See Liebel-Flarsheim Co., 358 F.3d at 911 (“[U]nless the court concludes, after applying all the available tools of claim construction, that the claim is still ambiguous, the axiom regarding the construction to preserve the validity of the claim does not apply.”).
IV
We agree with the district court’s construction of the “activator or inhibitor of a protein” limitation, and we therefore affirm the judgment of the district court.
COSTS
No costs.
AFFIRMED.